Cell marker |
CD3gamma, CD3delta, CD3ε, Rag1, Rag2, Ptcra, Tcf12, Ets, Ahr, Notch3, SpiB |
Evidence |
As genes specific for the DN2 stage, Cd117, Cd44, Hoxa9, Lmo2, Mef2c, Gfi1b, Lyl1, Spi1, Bcl11a, Mycn, and Hhex were used. Il2ra, Il7r, Erg, Notch1, Hes1, and Runx1 were used as genes expressed in both DN2 and DN3 cells. DN3 cells in particular were defined by high expression of CD3gamma, CD3delta, CD3ε, Rag1, Rag2, Ptcra, Tcf12, Ets, Ahr, Notch3, and SpiB. From the DN3 stage onward, Tcf12, Ets1, Bcl11b, Ets2, Lef1, Thy1, and Themis are highly expressed, whereas Cd4, Cd8, Rorc, and Id3 are increased once the cells have reached DN4 stage. |