| Evidence |
Te genes that characterized the B cell cluster included those involved in activation of the immune response (Iglc2), and antigen processing and peptide presentation (H2-Aa, H2-Eb1) in B lymphocytes.Pro-B, pre-B and B cells expressed Cd79a and Ebf1, which are required for B cell diferentiation, proliferation and signaling. Pro- and pre-B cells were marked by expression of Mzb1 and Vpreb3, which are involved in regulation of B cell proliferation and maturation. Dendritic cells were characterized by expression of Tcf4 which controls dendritic cell lineage specifcation10, Ccr9 which is involved in dendritic cell maturation11 and Siglech which is involved in mediation of the immune response12. Marker genes for monocytes included Fn1 and Ctss which are involved in monocyte diferentiation13, 14. Natural killer cells were characterized by expression of well-known marker genes Klrd1, Klrk1, Klre1 and Gzma15. Marker genes for HSPCs included Prtn3 and Lmo2 which regulate hematopoietic stem and progenitor cell proliferation16, Cdk6 which regulates hematopoietic and leukemic stem cell activation17, Cd34 which may be required for attachment to the bone marrow extracellular matrix and Ms4a3 which is involved in regulation of the cell cycle and known to be expressed in developing hematopoietic cells. T cells expressed known marker genes Cd3d and Trbc2. We conclude that in silico annotation of cell types with SingleR is accurate, as evidenced by expression of known marker genes, and these cell types will be used for subsequent downstream analysis. |
