| Cell marker |
Lyve1, Vcam1, Vcam1, Bmp2, Bmp2, C2, Cxcl1, Icam1, Cd274, Csf1, Il33, Ltb, Glycam1, Ccl20, Madcam1, Pd-l1, Cd74, Clu, Ifngr2, Mmp9, Relb, Msr1, Cxcl16, H2-Ab1, Pf4, Smad1, Smad4 |
| Evidence |
Figure 5. As noted earlier, the Mouse fLEC markers Madcam1 and Msr1 are poorly expressed by Human LECs. Figure 8. Ox1 shows reduced expression of fLEC marker genes Cd74 and Bmp2, and Ox2 of Ptx3-LEC marker genes including Stab2 and Tgfbi. Gray value intensity across the ROI for LYVE-1 (Marco-LEC marker) and MAdCAM-1 (fLEC marker) displaying decreasing or increasing expression along a path from the peri-follicular medullary sinuses to the SCS floor, respectively. fLECs are also enriched for chemokines, including the known fLEC marker Ccl20 (CCR6 ligand).As mentioned earlier, Bmp2 is specifically expressed by fLECs (Figure 5B and Figures S4, S5) that are also enriched for both Smad1 and Smad4 (Figure 5B and Figure S4) suggesting a role for BMP-signaling in fLEC homeostasis. We plotted gene expression of cells along the path from cLEC to fLEC (Figure S5 and Figure S7A) and found that individual cells within the bridge display variable levels of cLEC and fLEC marker transcripts including Lyve1. We plotted gene expression of cells along the path from cLEC to fLEC (Figure S5 and Figure S7A) and found that individual cells within the bridge display variable levels of cLEC and fLEC marker transcripts including Lyve1 (Figure S7A), but overall a decreasing gradient of cLEC markers (e.g., Ackr4 and Cav1) and an increasing gradient of fLEC markers (Madcam1, Pd-l1/Cd274) in the progression from cLEC to fLEC. However, RNAscope revealed that bridging cells lack mRNA for Bmp4 (cLEC marker) and have intermediate expression of Bmp2 (fLEC marker), a pattern consistent with the single cell profiles of the predicted bridge. |
