Cell marker |
Cd11c, CLEC10A, MhcII, CD14, CD209A, CLEC12A, CX3CR1, IL1A, P2RX7, NKG2D, MGL2 |
Evidence |
DCs defined as Lin(CD3, CD19, CD90)_ Ly6C_ CD64_ MHCII+ CD11c+, T-bet_ CD11b+ SIRPa+ cDC2s by 2 clusters (3 and 10). T-bet_ cDC2 clusters uniquely ex_x0002_pressed CLEC12A and could be further separated based on the expression of several cell surface markers by cluster 10 cells including CLEC10A (CD301a), CD209a, and NKG2D (Klrk1) (Figures 2A and S2C).Cd301b (Mgl2) was exclusively expressed by this cluster (Figure S2D) indicating that Mgl2+ cDC2s, previously reported in the skin and draining LN (Kumamoto et al., 2013), are akin to T-bet_ cDC2.Genes that defined T-bet_ cDC2s included Clec12a, Cx3cr1, Cd14, Il1a, and P2rx7 |