Evidence |
Using t-distributed stochastic neighbor embedding (tSNE) (10), global dimension reduction was constructed from all samples to visualize clusters that were defined by cell-specific gene markers (Figure 1A and Supplemental Table 2). / To identify specific markers for each cluster and consequently annotate them with the respective heart cell type, we applied Seurat “FindAllMarkers� function using the AUCbased scoring classifier with default parameters. We used the default Wilcoxon’s rank-sum test for the specific cell type analysis. |