| Evidence |
Two cell clusters were assigned as multipotent RPCs based on expression of multipotent RPC markers such as VSX2, HES1, and NR2E1, among others, as well as a large percentage of cycling cells (Figs. 4E, 4F).20 One other cluster was predominantly composed of cycling cells, with markers such as OLIG2, NEUROG2, OTX2, and ATOH7, which identify these as likely neurogenic RPCs39, 40 with limited mitotic potential. A second cluster had a smaller fraction of cycling cells, high ATOH7 levels, and expression of POU4F2, but no OTX2 or OLIG2, which we assigned to retinal ganglion cell (RGC)/amacrine (AC) RPCs and precursors as they exhibited markers of likely differentiation to RGCs or ACs |
