| Evidence |
Only 13.31% of ESC aggregates expressed PE markers, PDGFRa and Sox17.Frequency of ESC- or EPSC-derived blastoids (see STAR Methods) containing PE-like cells. 233 EPS-blastoids (n = 6) and 123 ES-blastoids (n = 3) werescored based on PDGFRa, alongside other PE markers including Sox17, Gata6, Gata4, and Foxa2.The proportionof DE and PE cells was then computed using the ‘WhichCells' function in Seurat v3 according to PE marker expression levels(Gata6 > 0.5 and Sox7> 0.5 and Rhox5 > 0.5) and DE cells on DE markers (Nnat>0.5 and Apela> 0.5 and Cer1 > 0.5) and dividingby the total amount of cells in the subsetted dataset. |
