| Evidence |
To validate these observations, we analyzed TE transcriptomes from single cells of blastoids at 96 h sorted using Cdx2 and Gata2 as respective polar and mural TE markers.Louvain clustering (Waltman and eck, 2013) delineated 3 distinct subpopulations within the TSCderived epithelium (Figure 4J). Cells in cluster 1 (Figure 4J; yellow), showed expression of self-renewal and mitotic cell cycle transcription factors, including Essrb, Eomes, Utf1, Ddah1, Pou3f1, Cpne3, markers of the polar TE. |
