Evidence |
The clustering result was annotated by the known markers including KRT12, KRT3 and GJA1 for differentiated cell; KRT14, KRT15 and KRT19 for progenitor; MKI67, BIRC5 and RRM2 for amplifying cell; TCF4, POSTN and A2M for LSC; TYR, TYRP1 and MLANA for melanocyte; and KRT4, KRT7 and KRT13 for conjunctival cells (Fig. 2F).C8 was the only cluster highly expressing major proliferation markers, including BIRC5, RRM2, TOP2A, MKI67 and PLK1 (Fig. 3A, C). This is consistent with the cell cycle analysis (Fig. 2D), which shows 98% of C8 cells in S and G2/M phases. Therefore, C8 is likely to be transiently amplifying cells (TACs) that are about to exit cell cycle and differentiate into corneal epithelial cells. |