| Evidence |
The clustering result was annotated by the known markers including KRT12, KRT3 and GJA1 for differentiated cell; KRT14, KRT15 and KRT19 for progenitor; MKI67, BIRC5 and RRM2 for amplifying cell; TCF4, POSTN and A2M for LSC; TYR, TYRP1 and MLANA for melanocyte; and KRT4, KRT7 and KRT13 for conjunctival cells (Fig. 2F).Based on the differentiation status the 5 differentiated cell clusters (C0_C4) can be divided into two subgroups (Fig. 3A and B). C0/2 were terminally differentiated cells (TDCs) as they expressed the highest levels of differentiation markers including corneal epithelial specific differentiation markers, cytokeratin 3 (KRT3) and KRT12, and keratinocyte cornification markers SPRR2A, SPRR1B and IVL, but did not express the proliferation and epithelial SC makers |
