| Evidence |
Two clusters predominantly present in the blood were na_ve CD4 (CD4 na_ve blood) and na_ve CD8 (CD8 na_ve blood) that were defined by expression of genes including CCR7, SELL, and LEF1. The blood also contained a cluster of CD4 T cells (CD4 memory blood) marked by expression of molecules associated with memory T cells (S100A4) and markers of various T cell subsets including the TH2 transcription factor GATA3 and the TH17 marker KLRB1 (CD161). Staining for the chemokine receptors CXCR3 that marks TH1 cells and CCR6 that marks TH17 cells, we find that CXCR3+CCR6_ and CXCR3+CCR6+ populations are the predominant memory CD4 T cell populations in the CSF, as the CSF contains significantly lower fractions of CXCR3_CCR6+ and CXCR3_CCR6_ cells than the blood |
