| Evidence |
To separate SSCs from progenitor cells among the group of spermatogonia at P10 and P15, we examined the expression of known SSC marker genes Id4, Gfra1, Lhx1, Egr2, Etv5, Nanos2, Ret, Eomes as well as progenitor markers Neurog3, Rarg, Nanos3, Lin28a, Upp135. For each cell, we calculated the fraction of SSC markers and progenitor markers expressed (>0 counts). The colour scale in Supplementary Fig. 5C indicates the fraction of SSC marker genes versus the fraction of progenitor marker genes expressed. |
