Evidence |
Violin plots demonstrate relative expression of these known SV cell type-specific genes across the four main cell types (Figures 2C, E). Confirmatory smFISH and immunohistochemistry demonstrates expression of these known markers for marginal cells (Kcne1, Kcnq1), intermediate cells (Cd44, Met), basal cells (CLDN11, ZO-1), and spindle/root cells (Slc26a4) (Figure 2F);Feature plots demonstrate expression of selected novel candidate genes in marginal cells (Abcg1, Heyl), intermediate cells (Nrp2, Kcnj13), basal cells (Sox8, Nr2f2), and spindle/root cells (P2rx2, Kcnj16) in both scRNA-Seq and snRNA-Seq datasets, respectively (Figures 3A, C) |