Evidence |
At the NMJ, specific myonuclei express AchR and AchE and at the MTJ-specific myonuclei express Col22a1;To look at the heterogeneity of the myonuclei, we have then reclustered the myonuclei expressing Titin (Ttn) gene to remove all the non-fiber nuclei (Fig. 1c).Several populations of myonuclei where identified, among which MTJ myonuclei (expressing Col22a1), NMJ myonuclei (expressing Ache), a yet unknown myonuclei subpopulation (expressing Myh9, Flnc, Runx1), and body myonuclei accounting for 94% of all myonuclei (Figs. 1c and 2a) |