Evidence |
As seen by UMAP, several clusters of nuclei were detected that express Ckm, a marker of mature myonuclei (Fig. 2 B and C and SI Appendix, Fig. S4B). These Ckm+ nuclei express different isoforms of myosin heavy chain (Myh), the classical marker for myofiber classification (19). We identified two large clusters of myonuclei with high levels of expression of Myh isoforms Cluster IIx expressed high levels of Myh1, which encodes myosin heavy chain protein IIx (MyHC-IIx), and Cluster IIb had high levels of expression of Myh4, encoding MyHC-IIb. |