| Evidence |
Cells in cluster MM_3 exhibited high_tga4, _y6c2, _cr2_nd_d62l(Sell)_xpression, but low_x3cr1_xpression (Supplementary Fig. 16A), resembling classical inflammatory monocytes (37_39). Cells in cluster MM_4 exhibited low_y6c2, _d62l(Sell)_nd_cr2_xpression, but high_tga4_nd_x3cr1_xpression (Supplementary Fig. 16A), consistent with a non-classical circulating monocyte phenotype (37_39). Cells in cluster MM_2 were characterized by the high_HC-II (H2.Aa, H2.Ab1, H2.DMb1, H2.Eb1), Aif1, Tmem176a, Tmem176b, CD86, Ass1_nd_xcl9_xpression (Fig. 6E), characteristic of M1-like macrophages._sf1a_eficiency and/or anti-PD1 treatment markedly expanded the cluster 2 population. Cells in cluster MM_5 were defined by high-expression of_rg1, Thbs1, Fn1 and Cd206 (Mrc1)(Fig. 6E), which was associated with immune-suppressive M2-like macrophages. Consistent with our previous observations, _sf1a_eficiency and/or anti-PD1 treatment reduced the cluster MM_5 population (Fig. 6D). |
