| Evidence |
Alveolar macrophages from cluster AM1 were characterised by expression of genes associated with normal homeostatic function of alveolar macrophages (Ear1 and Fabp1) (supplementary figure S4d). Macrophages from cluster AM2 expressed genes involved in the inflammatory response, cytokine production and matrix metalloproteinase activation (Car4, Ctsk, Chil3, S100a1 and Wfdc21) (supplementary figure S4d). In agreement with fate mapping studies, macrophages from cluster AM3 exhibited a more immature alveolar macrophage phenotype, characterised by lower expression of Pparg, Car4, Ear1, Siglecf and Marco, increased expression of Itgam, Cd36 and Gpnmb (supplementary figure S4c and d) |
