Evidence |
we identified six distinct clusters belonging to the epithelial (Epcam, Krt8 and Krt18), mesenchymal/neural crest (Lum, Col1a1 and Col3a1) and endothelial (Esam, Cav1, Igfbp7 and Sparcl1) cell lineages, as well as to innate immune (Coro1a, Fcer1g, Apoe, Cd52 and Cd68) and erythroid (Hba-a2, Snca and Alas2) cells , To validate the presence of these cell lineages at E16 using an orthogonal technique, we also identified epithelial (ECAD, EPCAM), mesenchymal (VIM), endothelial (PECAM1) and immune (F4/80) cell lineages by immunohistological analysis |