| Evidence |
The sodium phosphate cotransporter Npt2a was used as a robust PT marker (Figure 2B; Figure S1A), and the sodium chloride cotransporter (Ncc) used as a distal tubule/connecting tubule (DT/CNT) marker (Figure 2C; Figure S1B). Analysis of the single cell sequencing data confirmed that KL mRNA was highly expressed in the DT/CNT and moderately in the PT (Figure 2D, 2G). It also demonstrated that Hbegf (Figure 2E, 2H) along with the Hbegf receptor Egfr (Figure 2F, 2I) had higher expression in PT segments compared to other renal cell clusters. Thus, these results show that KL- and Hbegf-expressing cells localize to PT nephron segments |
