| Evidence |
we used tSNE projections and violin plots to identify the following types of innate immune cells in the Mouse kidney neutrophils (Lcn2+, Cluster 9, Figure 2A), NK cells (Gzma+ , Cluster 2, Figure 2B), ILCs (Il7r+ , Cluster 6, Figure 2C), infiltrating macrophages (Ccr2+, Itgam+ , Figure 2D), resident macrophages (Adgre1+, Fcgr1+, Cluster 0, Figure 2E), dendritic cells (Snx22+, Batf3+ , Clusters 1 and 7, Figure 2F), and unknown (Cluster 5). Additional mark ers including Plac8, Lyz2, and Cebpb were used to identify in- filtrating macrophages as clusters 3 and 4 (Figure 1).23 We also identified a small population of B cells (Cd79a+ , Cluster 8, Supplemental Figure 1) in our lineage-negative scRNAseq data, despite the use of a pan B cell marker (B220). |
