| Cell marker |
Cldn5, Fabp4, Cav1, Stab2, Cd9, Mmrn1, Mmrn2, Cd93, Pdgfa, Cdh13, Edn1, Fn1, Klf4, Nts, Ackr4, Bmp4, Jag1, Pdgfb, Gdf10, Gja4, Nudt4 |
| Evidence |
Figure 5. Figure 8. Deletion of Foxc2 in lymphatic vessels has been shown to lead to downregulation of the cLEC marker Ackr4, but also to mislocation of surrounding smooth muscle cells. We plotted gene expression of cells along the path from cLEC to fLEC (Figure S5 and Figure S7A) and found that individual cells within the bridge display variable levels of cLEC and fLEC marker transcripts including Lyve1 (Figure S7A), but overall a decreasing gradient of cLEC markers (e.g., Ackr4 and Cav1) . However, RNAscope revealed that bridging cells lack mRNA for Bmp4 (cLEC marker) and have intermediate expression of Bmp2 (fLEC marker), a pattern consistent with the single cell profiles of the predicted bridge. |
