| Evidence |
our results identified monocyte (Cd14, Fcgr3), macrophage (Adgre1, Csf1r), dendritic cell (Flt3), T cell (Cd3g, Cd4, Cd8b1) and natural killer cell (Ncr1), B cell (Cd19, Cd79a, Jchain) and plasma cell (Jchain, Sdc1), Endothelial cells (Pecam1, Cdh5), and mesenchymal stromal/stem cell (MSC, expressing Lum, Dcn, Cd34, Pdgfra) (Online Figure III).we detected four distinct DC subpopulations (Figure 2G). Cluster 9, characterized by high expression of Cox6a2 and Siglech, was suggested to be plasmacytoid DC, a principal type I interferon-producing cell in the body26. Cluster 10 was most similar to CD209+ monocyte derived-DC27, with expression of Cd209a, Clec10a and monocyte markers CD14 and Fcgr3 (Figure 2C). Cluster 11 was enriched for DC maturation and migration marker Cacnb3, Fscn1, Ccr7, suggestive of CCR7+ migratory DC28, a population homing to the lymphatics. Cluster 12 was closest to XCR1+ cross-presenting DC29, in accordance with high expression of Xcr1. |
