| Evidence |
Differentially expressed genes (DEGs) in cluster 0 comprised several genes associated with lymphocyte activation and T cell differentiation (Fig. 2b), including , Pdcd1 (encoding PD-1), Cd27, Cd28, Cd69, Slamf6, Cd81, Ldhb and Bcl2 (coding for the anti-apoptotic protein BCL-2) (Fig. 2d). Several genes coding for transcription factors, such as Lef1 (ref. 19), Sox4 (ref. 20), Egr2 and Egr1 (ref. 8), Tox21, Id3 (ref. 22), Myb23, Ikzf1 and Ikzf2 (coding for IKAROS and HELIOS, respectively), including some that have been previously implicated in iNKT cell development, were highly expressed in stage 0 iNKT cells |
