| Cell marker |
Pecam1, Cldn5, Flt1, and Cdh5, Nrp1, Dll4, Rcan2, Angpt2, Prdm1, Hdac9, Pde4b, Arhgap18, Itga4, Rgcc, Cxcl12, Rasgrp3, Akr1c14, Slc25a33, Smarca2, Ets2, Sema6d, Gja1, Enpp2, Col4a3, Cdc42ep3, Bcl2, Htra3, Gpr85, Cyp2d22, Trak2, Camk2n1, Nid2, Lrrn3, Plekhh2, Stra6, Rgl1, Pcx, Slc1a1, Osgin1, Klhl6, Spry1 |
| Evidence |
The ECs had high expression of known EC-specific marker genes (e.g. Cldn5, Flt1, Pecam1, and Cdh5).We employed a probabilistic method42 (see Methods section) to assign each of the ECs from young adult (n = 8627 cells) and aged (n = 3730 cells) Mouse brains to one of six molecular subtypes with distinct arteriovenous distributions based on the expression profiles of 267 arteriovenous zonation-dependent genes in ECs13 (Fig. 1c, Supplementary Fig. 1b, and also see Supplementary Table 1 for lists of the genes). |
