| Evidence |
The cell cluster C7 included cells expressing genes associated with nonmyelinating Schwann cells (SCs) and SCs that have lost contact with axons (e.g., Gap43, Ngfr, Bdnf, and Stmn1). Cells in cell cluster C9 expressed markers of myelinating SCs (e.g., Plp1, Qk, and Vim) but cells that expressed the immature SC marker Fabp7 (Blbp). Cxcr3 was not detected in any cells in this analysis of 2-month-old Mouse. Next, we examined expression of Cxcl10 in these clusters. As visualized by t-distributed stochastic neighbor embedding (t-SNE) plots, Cxcl10 expression localizes to cell cluster C9 (labeled SC-2) (Figure 2B). To further explore Nf1 and Cxcl10 expression, we plotted their relative expression in individual cells in SC-1 (C7) and SC-2 (C9) (Figure 2C). Although 26.2% of cells in SC-2 retained Nf1 expression and expressed Cxcl10 (red dots within box in Figure 2C), in general, Nf1 is expressed in cells in SC-1 (blue dots along y axis); most Cxcl10-expressing cells in SC-2 (red dots along x axis) have low or undetectable Nf1. |
