| Evidence |
an Activated Macrophage cluster defined by enrichment of interferon-stimulated genes such as Ifit3 and Isg15 (Cluster 14), a “Trem2-hi Macrophage� cluster defined by high expression of Trem2 and C1qa (cluster 12), and an “Inflammatory Macrophage� cluster defined by high expression of Il1b (Cluster 8) (Figure 5A). To understand how Tregs affect these macrophage subpopulations in regressing plaques, we performed differential gene expression and gene ontology enrichment analyses of plaque macrophage subsets from mice at baseline and under regression conditions with IgG or PC61 mAbtreatment. Of the 4 macrophage subsets examined, the presence of Tregs prominently affected the transcriptional profile of activated macrophages (Figure 5B). Compared to baseline, activated macrophages in regressing plaques of IgG-treated mice showed an increase in expression of genes associated with a tissue reparative M2-like macrophage phenotype, including Il4ra, Il10ra, Stat3 and Stat6, genes involved in JakStat signaling (Jak1, Stat1), as well genes involved in efferocytosis (Rac1, Il10ra, Mfge8, Mafb, Fasl) (Figure 5B) |
