| Evidence |
Canonical mesenchymal progenitor markers_d34, _dgfra, _y6a(Sca1), and_hy1(Cd90) were expressed predominantly in groups 1 to 3 (figs. S2 and S3). Group 1 cells (blue), which we named "interstitial progenitors, " were marked by the expression of_pp4_encoding dipeptidyl peptidase_4 (DPP4)], _nt2, _mp7, and_i16_ut did not express adipocyte markers (Fig. 1B_nd figs. S2 and S3). Group 2 cells expressed_cam1_encoding intercellular adhesion molecule_1 (ICAM1)] and_lk1(Pref1), along with several adipocyte identity genes, including_parg, _abp4, and_d36, suggesting that these cells could be "committed preadipocytes" (Fig. 1B_nd figs. S2 and S3). Group 3 cells expressed_lec11a_nd_mo2, as well as tissue factor (F3/CD142), which is a marker of "adipogenesis-regulatory cells" (Aregs) that were recently reported to inhibit adipogenesis (24). A related cell cluster (group 4) was marked by_nt6_nd_frp5_xpression but did not show detectable expression of mesenchymal marker genes, such as_dgfra_r_hy1._roup 7 cells (yellow) were classified as "adipocytes" because of their high expression of mature adipocyte_specific genes (e.g., _dipoq, _lin1, _nd_ar3) and lack of expression of progenitor markers. |
