| Evidence |
We used t-distributed stochastic neighbor embedding (t-SNE) visualization of cells to reveal 10 major clusters (Figure 1A), based on marker gene expression for pDCs (e.g., enrichment of_LEC4C, _CF4) (25), mDCs (CD1C, _PI1) (26), macrophages (CD68, _D14) (27), NK cells (NKG7, _RF1) (28), ILCs (IL7R) (29), IDO+_yeloid cells (IDO1), mast cells (TPSAB1, _TSG) (30), CD34+_rogenitor cells (CD34), B cells (CD79A, _D19, due to incomplete depletion by the CD19 antibody), and erythroid cells (HBB, _BA2) (31), which may not have been completely lysed by the red blood cell lysis procedure (Figure 1, B and C; and_upplemental Table 1; supplemental material available online with this article;_ttps |
