| Cell marker |
CD1C, CD1C, CLEC9A, CLEC9A, CLEC4C, IL3RA, LILRA4, CD1E, CD1E, CPVL, LAMP3, PTPRC, FCGR2A, CD123, KIAA0101, TLR7, TLR7, CXorf21, MCOLN2, CX3CR1, ITGAD, CX3CR1, ITGAD, CD11D, CD45, CD55, COTL1, CYTIP, DDIT4, PPIF |
| Evidence |
Upon reanalysis of this cluster, we identified three macrophage subsets, marked by high expression of CCR1, MARCO or TREM2; and six dendritic cell subsets, marked by high expression of CLEC9A, CXorf21, MCOLN2, LAMP3, KIAA0101 and Langerin, representing respectively cDC1, two subsets of cDC2, a novel DC type, a cluster of proliferating DC, and a Langerhans cell subset.Other macrophage markers like CD163, LILRB4, CSF1R (CD115), CD86, TNFSF18, CD80, CD33, FCGR1A (CD64A), FCGR1B (CD64B) and FCGR2A (CD32) were also selectively expressed by cells in cluster 11.However, some described markers for macrophages like LGALS3, used to identify macrophages in IHC and TFRC (CD71), were expressed by cells in almost every cluster. Figure S6.Fig.4a.we found immune mediators CXCL1, CXCL2, CXCL3, CCL2, CCL4 highly expressed in Mw/DC subcluster 2; and Mw markers CD14, SEPP1 and genes encoding for serum complement subcomponent C1q highly expressed in Mw/DC subcluster 3.Compared with our data, MARCO Mws were similar to aortic resident-like Mw with common highly expressed genes including F13A1, FOLR2, TXNIP and SEPP1, especially TXNIP and SEPP1 have been identified as markers of resident Mws. |
