| Evidence |
To assess the identity of the cell type in each cluster, we examined the expression of PROX1, CCL19, and JAM2 expressed specifically in LECs (Wigle and Oliver, 1999), non-endothelial stromal cells (SCs) (Chai et al., 2013), and blood Endothelial cellss (BECs) (Aurrand-Lions et al., 2001) respectively (Figure 1C). PROX1 was expressed on clus_x0002_ters 1_6 (LEC I_VI), whereas JAM2 or CCL19 were expressed on clusters 7 and 9 or clusters 8 and 9, respectively. Cluster 9 expressed both SC and BEC markers (Figures 1C and 1D), resembling CD34+ SCs, which have been recently described in the Mouse LNs by scRNA-seq |
