| Evidence |
These cells could be assigned to ten distinct cell types (Fig. 1B) using known marker genes malignant cells (11, 601 cells, 37.1%, marked with EPCAM, keratin 19 [KRT19], and KRT7); cholangiocytes (546 cells, 1.7%, marked with FYXD2, TM4SF4, and ANXA8); heocytes (328 cells, 1.0 %, marked with APOC3, FABP1, and APOA1); B cells (827 cells, 2.6%, marked with MS4A1 and CD79A); T cells (10, 883 cells, 34.8%, marked with CD2, CD3D, and CD3E); natural killer (NK) cells (1, 597 cells, 5.1%, marked with CD7, FGFBP2, and KLRF1); macrophages (3, 407 cells, 10.9%, marked with CD14); dendritic cells (DCs; 765 cells, 2.4%, marked with CLEC9A and CD1C); fibroblasts (498 cells, 1.59%, marked with ACTA2 and COL1A2) ; and Endothelial cellss (ECs; 823 cells, 2.6%, marked with ENG and vWF; Fig. 1C, Table S2). |
