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ID 2886
Class Mammalia
Species Homo sapiens
Tissue Kidney
Sample Organoids
Disease type Kidney_umor
Cell type Proximal tubular cells
Cell type (subtype) Proximal tubular cells
Cell marker LRP2, SPP1
Frequency
PubMed ID 31784515
Journal Nat Commun
SCI IF 12.121
Evidence D29 organoids from all four iPSC lines contained cells representative of segments of a developing nephron (Fig.1c, d_nd Supplementary Fig.2).ascending limb (TAL;_LC12A1, Na-K-Cl cotransporter), and distal nephron cells (CDH1, E-cadherin;_QP2, aquaporin 2;_ATA3). There was no cluster enriched for_LC12A3(Na-Cl symporter; Supplementary Fig.2), a canonical marker of the distal convoluted tubule (DCT). The organoid single-cell profiles retained the proximal (podocyte) to distal axis of the Human nephron (Fig.1c, left) on visualization of the data using t-distributed stochastic nonlinear embedding (tSNE), unlike the discrete clusters seen in adult kidney (Fig.1c, right). We identified data-derived markers (Supplementary Table2), including osteopontin (SPP1)22_n the proximal tubular cell cluster, and_FDC223_nd_AL24_n the distal nephron cluster (Fig.1d_nd Supplementary Fig.3A), in line with scRNA-seq studies of Human adult and fetal kidney.D29 organoids also contained nephron progenitor cells (NPC) enriched in PAX2, LHX1 and PAX8
Title Single cell census of Human kidney organoids shows reproducibility and diminished off-target cells after transplantation
Authors Ayshwarya Subramanian et al.
Date 2019.11
Data available GSE136314