| Evidence |
we identified alphabeta T cells (CD3E, LCK, TRAC, and TRAJ16) subsetting into CD4+ T cells(IL7R and CD4), activated CD8+ T cells (CD8a; CD8B and CCL5), nonactivated CD8+ T cells (CD8na; CD8B and CCR7), regulatory T cells (FOXP3 and CTLA4), and a small cluster of gammadelta T cells (TRDC). Two natural killer (NK) cell clusters (GNLY and NKG7) most likely represented the more cytotoxic and mature CD56dim (NK1; FCGR3A/CD16 and PRF1), and more naive CD56bright (NK2; SELL/CD62L and XCL1) subsets. Three B lineage clusters (CD74, CD79A, and IGH gene family) corresponded to naive B cells (B1; CD37 and IGHD), activated B cells (B2; CD27 and IGHM), and plasma blasts (plasma; IGHG, CD38, and TNFRSF17/CD269; negative for MS4A1/CD20 and SDC1/CD138). Myeloid lineage cells (LYZ) separated into mDC type 1(mDC1; WDFY4, XCR1, and BATF3), mDC type 2 (mDC2;FCER1A, CD1C, and CLEC10A), and granulocytes (granulo;S100A8 and S100A9). Two additional monocyte cell clusters were mostly blood-derived (Mono1; FCGR3A/CD16) or CSF-derived (Mono2; CD14). Additional clusters represented plasmacytoid dendritic cells (pDC; TCF4/E2-2 and TNFRSF21/DR6) and megakaryocytes (MegaK; GNG11 and CLU). |
