| Evidence |
which we subsequently categorized into the following six major cell types according to their individual transcriptome profiles and previously reported cell-type markers: astrocytes (AQP4+, 11.9 ± 1.4% of total nuclei), Endothelial cellss (CLDN5+, 2.3 ± 0.5%), excitatory neurons (CAMK2A+, 45.2 ± 1.7%), inhibitory neurons (GAD1+, 14.1 ± 0.9%), microglia (C3+, 4.7 ± 0.6%), and oligodendrocytes (MBP+, 21.8 ± 2.5%) (Fig. 1 B and C and SI Appendix, Fig. S1 C and D). In addition to the previously reported cell-type markers, these six major cell types expressed the following unique signature genes, which can serve as novel cell-type markers ADGRV1, GPC5, and RYR3 were expressed by astrocytes; ABCB1 and EBF1 by Endothelial cellss; CBLN2 and LDB2 by excitatory neurons; LHFPL3 and PCDH15 by inhibitory neurons; LRMDA and DOCK8 by microglia; and PLP1 and ST18 by oligodendrocytes |
