| Evidence |
We found that ISL1/LHX3 alone were insufficient to induce neuronal morphologies (Figure 3A) and eliminate S100A4 (a fibroblast marker also known as FSP1) and induce a neuronal marker MAP2 by day 22 in contrast to cells expressing miR-9/9_-124 only (Figures 3B and 3C). ells immunostained for fibroblast identity marker FSP1 (green) and morphology marker TUBB3 (red) over DAPI (blue). Examining promoter and intragenic chromatin accessibility correlated to gene expression, we identified about 200 genes that miR-9/9_-124 differentially closed and downregulated (Log2FC ≤ _1, adj. p < 0.01) including canonical fibroblast fate markers, S100A4, FBLN1, MFAP5, and COL1A1 (Figure 3F; Figure S4C). |
