| Evidence |
Also, as alternative fates have been previously identified in neuronal conversion methods based on ASCL1 (Herdy et al., 2019; Treutlein et al., 2016), we assayed miNs and moto-miNs for myocyte and stem cell markers. Interestingly, we did not detect myocyte (MYH3) or stem cell (SOX2) markers in moto-miNs. Similarly, we detected no induction of myocyte cell clusters and markers, including ACTA1, MYOG, MYOD1, and MYO18B in miNs by scRNA-seq, or the upstream ASCL1, likely accounting for the absence of the alternative myogenic induction (Figure S2E). |
