Cell marker |
CD8, NKG7, GZMB, LAG3, PDCD1, GZMH, GNLY, FGFBP2, HLA-G |
Evidence |
Although the composition of the cells was largely similar, we identified one cluster (‘cluster 0') which was enriched in Pt. 4. Examination of genes differentially expressed in this cluster of cells suggested a cellular identity concordant with that of highly cytotoxic memory (TBX21-expressing) T cells, which had an abundance of MHC-I (HLA-A/B/C) and MHC-II (e.g., HLA-DRA, HLA-DRB5) family member expression as well as expression of cytolytic and immune checkpoint genes;Seven genes enriched in cluster 0 (PDCD1, NKG7, LAG3, GZMH, GZMB, GNLY, FGFBP2) and one gene strongly de-enriched in patient 4(HLA-G) were chosen as an 8 gene signature for downstream applications, including validation in a larger cohort of patients; |